Senior RA/Principal RA/Scientist Molecular Biology and Cell Engineering

Senior RA/Principal RA/Scientist Molecular Biology and Cell Engineering

Dragonfly Therapeutics is seeking a highly motivated and experienced senior/principal research associate/scientist with a strong focus on molecular biology, mammalian cell culture, viral transduction, and flow cytometry to join the Biologics team in Copenhagen, (DK). The successful candidate will be a key member of the antibody discovery group responsible for design of plasmids and cloning, viral transduction and cell line development, CRISPR-mediated gene manipulation, and flow cytometric evaluation.


Responsibilities

  • Vector Design and cloning of plasmids for expression of recombinant proteins, antibodies and TriNKETs
  • Virus-mediated transduction and creation of isogenic cell lines expressing surface proteins
  • Cell-based screening and Flow cytometric evaluation and characterization of antibodies/TriNKETs
  • CRISPR/Cas9 mediated manipulation of cell lines for gene knock-in or knock-out
  • Molecular biology including sequence analysis, qPCR, RT-PCR, restriction digest and gel electrophoresis
  • Mammalian cell culture and high throughput protein production
  • Interface with CROs for sequence analysis, DNA scale-up, and cell line development
  • Generate, manage, analyze, and maintain critical data in a highly organized manner

Qualifications

  • B.S or M.S. with 5+ years of industry experience or Ph.D. in cell line development and characterization (title and salary commensurate with experience)
  • Extensive experience in vector design, Gibson, Golden Gate and restriction cloning, and creation of plasmids for expression of recombinant proteins and antibodies/TriNKETS
  • Proficiency in design and creation of retroviral/lentiviral plasmids, transduction of cell lines and primary cells
  • Hands-on experience in CRISPR/Cas9 mediated gene manipulation
  • Proficient in Flow Cytometric evaluation and characterization of cell lines for antibody binding and potency
  • Experience in extraction of immune cells from blood and their vitro characterization will be preferred
  • Experience in protein expression using Expi293 and ExpiCHO cells is a plus
  • Excellent aseptic skills, and mammalian cell culture experience is a must.
  • Hands-on experience with standard molecular biology skills including bacterial culture and transformation, qPCR, RT-PCR, DNA purification, restriction digestion, sequence analysis and vector construction.
  • Experience working with CROs for sequence analysis, DNA scale-up and cell line development
  • Efficient time management and ability to work on several projects simultaneously
  • Strong interpersonal skills and ability to effectively communicate with members of the multidisciplinary team
  • The ability to work collaboratively, provide strategic input, communicate clearly both written and orally is critical for success in this position.
  • Excellent record keeping abilities, to document analytical data generated in support of regulatory requirements, adherence to GDP, experience with ELN

About Dragonfly

Founded in 2015 by Dr. Tyler Jacks, head of the Koch Institute at MIT, Dr. David Raulet, one of the world's leading experts in Natural Killer (NK) cell biology, and Bill Haney, a longtime tech entrepreneur and investor, Dragonfly Therapeutics was launched to harness the power of the immune system to provide breakthrough treatments for patients – especially in areas where there are no effective treatments, today.


The company develops novel first-in-class therapeutics designed to harness Natural Killer cells and other cells of the innate immune system which can provide direct killing of disease promoting cell types, and provide a unique therapeutic window beyond current therapies for treatment of cancer and chronic inflammatory diseases. Please visit our websitefor details.


We offer

Our employees are fundamental to our success, and we continue to be able to attract and retain people with different experiences and backgrounds. We have a high-performing, collaborative culture characterized by respectful and diverse teams who are committed to our patients first mission.


At Dragonfly, you will have the opportunity to have impact, work with some of the brightest minds in the industry, develop your professional career, and contribute to breakthrough medicines for patients with urgent unmet medical needs.


Your application

Please apply by sending a cover letter and resume as soon as possible through the button "Apply / Send ansøgning". We are screening and interviewing for the position on an ongoing basis. For any further questions please email hr@dragonflytx.com


For more info, check out our career site https://dragonflytx.ofir.com


Du har ikke vedlagt dokumenter til din ansøgning. Klik på 'OK' knappen nedenfor for at sende din ansøgning alligevel, eller klik på knappen "Annuller" og vedhæft dokumenter, før du sender din ansøgning igen.

Modtag jobannoncer der minder om dette job i din indbakke.Når du tilmelder dig accepterer du samtidig vores privatlivspolitik. Du kan altid trække dit samtykke tilbage.

Mere af samme slags?

Modtag jobannoncer der minder om dette job i din indbakke.




Når du tilmelder dig accepterer du samtidig vores privatlivspolitik. Du kan altid trække dit samtykke tilbage.

Andre job der matcher din søgning

Senior Research Associate/Scientist, Protein Expression and purification

Dragonfly Therapeutics CPH ApS

Ballerup
Senior Research Associate Scientist, Protein Expression and purification We are looking for a highly motivated and talented biochemist to join our Biologics team in Copenhagen, (DK). The successful candidate will be responsible for performing clonin...
Indrykket:26. november 2020

Molekylærbiolog (cand.scient. eller tilsvarende), Herlev- og Gentofte Hospital

Herlev Hospital

Herlev
En fuldtidsstilling som fondsansat forsker molekylærbiolog ved MolekylærEnheden, Patologiafdelingen, Herlev Hospital, er ledig til besættelse pr. 1. januar 2021 eller snarest derefter. Stillingen besættes for ét år med mulighed for forlængelse. Fors...
Indrykket:20. november 2020

Postdoc Position in RNA Biology and Adipose Metabolism

Rigshospitalet

København Ø
Center for Physical Activity Research Rigshospitalet, University of Copenhagen We are looking for a postdoc with a passion for science and experience in RNA biology and or organelle communication to start January 4th, 2021 or according to agreement....
Indrykket:19. november 2020

In Vitro Scientist Within Atherosclerosis for Cardiovascular Research

Novo Nordisk A/S

Måløv
Novo Nordisk - Research Development Denmark - Måløv We are seeking a talented scientist to be part of Novo Nordisk A S and participate in changing the quality of life for millions of people with cardiovascular diseases. About the department Heart Va...
Indrykket:19. november 2020

Senior Researcher in the field of fungal natural products

DTU

Kongens Lyngby
The Yeast Natural Products Section at the Center for Biosustainability is seeking a dedicated senior researcher (co-Principle Investigator) to lead a team of scientists and engineers to engineer yeast to produce a variety of natural and unnatural pr...
Indrykket:16. november 2020

Mere af samme slags?

Modtag jobannoncer der minder om dette job i din indbakke.




Når du tilmelder dig accepterer du samtidig vores privatlivspolitik. Du kan altid trække dit samtykke tilbage.

Andre job der matcher din søgning

Senior Research Associate/Scientist, Protein Expression and purification

Dragonfly Therapeutics CPH ApS

Ballerup
Senior Research Associate Scientist, Protein Expression and purification We are looking for a highly motivated and talented biochemist to join our Biologics team in Copenhagen, (DK). The successful candidate will be responsible for performing clonin...
Indrykket:26. november 2020

Molekylærbiolog (cand.scient. eller tilsvarende), Herlev- og Gentofte Hospital

Herlev Hospital

Herlev
En fuldtidsstilling som fondsansat forsker molekylærbiolog ved MolekylærEnheden, Patologiafdelingen, Herlev Hospital, er ledig til besættelse pr. 1. januar 2021 eller snarest derefter. Stillingen besættes for ét år med mulighed for forlængelse. Fors...
Indrykket:20. november 2020

Postdoc Position in RNA Biology and Adipose Metabolism

Rigshospitalet

København Ø
Center for Physical Activity Research Rigshospitalet, University of Copenhagen We are looking for a postdoc with a passion for science and experience in RNA biology and or organelle communication to start January 4th, 2021 or according to agreement....
Indrykket:19. november 2020

In Vitro Scientist Within Atherosclerosis for Cardiovascular Research

Novo Nordisk A/S

Måløv
Novo Nordisk - Research Development Denmark - Måløv We are seeking a talented scientist to be part of Novo Nordisk A S and participate in changing the quality of life for millions of people with cardiovascular diseases. About the department Heart Va...
Indrykket:19. november 2020

Senior Researcher in the field of fungal natural products

DTU

Kongens Lyngby
The Yeast Natural Products Section at the Center for Biosustainability is seeking a dedicated senior researcher (co-Principle Investigator) to lead a team of scientists and engineers to engineer yeast to produce a variety of natural and unnatural pr...
Indrykket:16. november 2020